ABSTRACT
OBJECTIVE@#To study the changes of HSP 70 mRNA and c-fos mRNA expression and to find a method to differentiate antemortem from postmortem electrocution.@*METHODS@#Fifteen New Zealand rabbits were randomly divided into three groups, the antemortem electrocution group, the postmortem electrocution group, and the control group. Each group consists of five rabbits. The levels of HSP 70 mRNA and c-fos mRNA in skeletal muscle and cardiac muscle were examined with quantitative fluorescent RT-PCR.@*RESULTS@#The levels of HSP 70 mRNA and c-fos mRNA in the antemortem electrocution group increased significantly (P<0.05), compared with that of the postmortem electrocution group.@*CONCLUSION@#The changes of HSP 70 mRNA and c-fos mRNA expression in skeletal muscle and cardiac muscle can be used as an indicator to distinguish antemortem from postmortem electrocution.
Subject(s)
Animals , Male , Rabbits , Electric Injuries/metabolism , Forensic Pathology , HSP70 Heat-Shock Proteins/metabolism , Muscle, Skeletal/metabolism , Myocardium/metabolism , Postmortem Changes , Proto-Oncogene Proteins c-fos/metabolism , RNA, Messenger/metabolism , Random AllocationABSTRACT
<p><b>OBJECTIVE</b>To study the preventing effects of procyanidins (PC) on selenite cataract in rats and the time-effect relationship.</p><p><b>METHOD</b>Forty five SD rats were divided into three groups: control, model and experiment groups. The rats in the experiment group were fed additionally with the PC by 80 mg x kg(-1) when they were supplied the equal selenite with the model group. Five rats of each group were regularly sacrificed by bleeding from femoral artery at sixth, eleventh, sixteenth day and the level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of all lenses was measured.</p><p><b>RESULT</b>Compared with the model group, the level of the MDA in the experiment group at the eleventh day and the sixteenth day greatly decreased (P < 0.01). At the sixteenth day the level of the SOD and GSH-Px had an increase (P < 0.01), which showed its anti-oxygenation.</p><p><b>CONCLUSION</b>PC indicated the obvious inhibition in the development of the rat cataract. The treatment period was recommended at least for fifteen days.</p>
Subject(s)
Animals , Female , Male , Rats , Cataract , Glutathione Peroxidase , Metabolism , Malondialdehyde , Metabolism , Proanthocyanidins , Therapeutic Uses , Random Allocation , Rats, Sprague-Dawley , Sodium Selenite , Pharmacology , Superoxide Dismutase , MetabolismABSTRACT
Objective To investigate the effect of perinatal bisphenol A BPA exposure on brain development of F1 male offspring. Methods Pregnant SD rats were given BPA at 2 20 and 100 mg/kg body weight per day respectively from eleventh day of gestation to the whole lactation by gavage until their pups were weaned on postnatal day 21 the control group had no BPA exposure. Every six F1 male pups from each of the four groups were killed at differential time points on postnatal day 1510152130 and 45 respectively. Histopathological examination by HE stain was done on the brains. Results The results showed no abnormal change was found on postnatal day 1-10. Three dosage groups showed abnormal change of different degree on 15th 21th 30th postnatal day the mainly abnormal change was karyopyknosis of pyramidal cell in CA3 of hippocampus and cortical neuron in cerebral cortex. The cell numbers of pyramidal cell in CA3 of hippocampus and cerebral cortex were decreased on 45th postnatal day. Conclusion Perinatal BPA exposure may have an adverse effect on the brain developmnent of F1 male offspring.